Semiquantitative reverse transcription-polymerase chain reaction analysis of syndecan-1 and -4 messages in cartilage and cultured chondrocytes from osteoarthritic joints

摘要: Abstract Objective To determine the steady-state of messenger RNA (mRNA) levels syndecan-1 and syndecan-4 in cartilage samples chondrocytes derived from human osteoarthritic knee joints. Methods Steady-state gene-specific mRNA (relative to β-actin) were measured by semiquantitative polymerase chain reaction (PCR). Results RT-PCR allowed detection (for first time) both articular cultured as primary monolayers. The reduced tissue heavily damaged compared normal-looking areas whereas those significantly increased. In contrast, expression was higher fibrillated than cells obtained intact cartilage, while message did not differ between two sites. Conclusion cell-surface syndecans 1 4 is altered during degradative process joint. discoordinate syndecan gene expression, which probably related chondrocyte proliferation clustering, may contribute disorganization development OA processes. Isolation culturing monolayers dramatically change these genes cannot reflect situ condition.