Immunoadsorption of IgG onto second antibody covalently attached to Amino-Dylark beads for radioimmunoassays.

摘要: We present a solid-phase immobilization method for radioligand assays, using an immunoadsorption coating procedure of anti-triiodothyronine rabbit IgG (anti-T3 IgG) onto second antibody (sheep anti-rabbit covalently bound to Amino-Dylark beads. The was in excess, compared with the first antibody, thus eliminating reproducibility problems between immunoadsorptions. Beads coated can be used immobilize variety antigen-specific antibodies. amount anti-T3 required T3 radioimmunoassay (RIA) only 10% more, per assay tube, than that utilized liquid-phase RIA, which polyethylene glycol solution separation reagent; characteristics performance were comparable. requires high-titer antisera or and seems advantageous because decrease requirements without significant modification functionality.