Immobilization of immunoglobulins onto surface-treated and untreated polystyrene beads for radioimmunoassays.

摘要: The immunoreactivity of anti-triiodothyronine (anti-T3) IgG, pretreated at acidic pH and adsorbed onto polystyrene beads, was significantly greater than that determined for native anti-T3 IgG immobilized optimum 3.5. Acidic-pH-pretreated 7.0 ethanol-treated had less on untreated beads gave values similar to those activated 9.6. rate immobilization treated ones, the binding reproducible (intra- inter-batch coating CV, 3.7-4.6% 7.2%, respectively) very resistant successive washings. A post-washing incubation in 10 mg/L bovine serum albumin solution required eliminate decreased caused by some washing reagents. For solid-phase radioimmunoassays T3, 40% antibody needed when acidic-pH-pretreated or comparison with beads. Results assay characteristics such were comparable results liquid-phase polyethylene glycol T3.