Germ line transmission of an inactive N-myc allele generated by homologous recombination in mouse embryonic stem cells.

摘要: Abstract We have disrupted one allele of the N-myc locus in mouse embryonic stem (ES) cells by using homologous recombination techniques and obtained germ line transmission null ES cell lines with to offspring. The creation mice a deficient will allow generation offspring bearing alleles both chromosomes permit study role that this proto-oncogene plays development.