Distribution and persistence of technetium-99 hexamethyl propylene amine oxime-labelled bone marrow-derived mesenchymal stem cells in experimentally induced tendon lesions after intratendinous injection and regional perfusion of the equine distal limb.

摘要: Reasons for performing studyIntralesional (i.l.) injection is currently the most commonly used technique stem cell therapy in equine tendon injury. A comparison of different techniques mesenchymal cells treatment lesions required.ObjectivesWe hypothesised that vascular perfusion distal limb with (MSCs) would result preferential distribution MSCs to acute injuries.Study designIn vivo experimental study.MethodsLesions were surgically induced forelimb superficial digital flexor tendons 8 horses. Three or 10 days after lesion induction, technetium-99 hexamethyl propylene amine oxime-labelled injected via i.v. intra-arterial (i.a.) regional (RLP) at level antebrachium and compared i.l. injection. Mesenchymal persistence within was assessed scintigraphy 24h.ResultsLesion uptake higher than RLP, but MSC decreased similarly over time all 3 techniques. Intra-arterial RLP resulted a better site RLP. Limbs perfused i.a. on Day showed greater accumulation limbs 3. Arterial thrombosis occurred 50% 100% limbs, which led clinical complications one horse.Conclusions potential relevanceCompared injection, results lower similar lesions. dependent identified The appears more advantageous terms uptake. However, described produced arterial thus cannot be recommended use. Summary available Chinese - see Supporting information.