THE ROLE OF CERULOPLASMIN IN COLITIS

摘要: Ceruloplasmin (Cp) is an acute phase, plasma protein with multiple enzymatic activities consistent both proand anti-inflammatory functions. Our laboratory has recently reported the presence of Cp in intestinal epithelial cells. To determine potential role inflammation, we induced colitis Cp-deficient mice by continuous administration dextran sodium sulfate (DSS) ad libitum drinking water. The Cpnull rapidly lost weight and all were moribund day 14, while about 90% wild-type (WT) survived at least 20 days. Higher amounts TNF-, neutrophil (KC) macrophage (MCP-1) chemokines, detected colon culture supernatants Cp-null compared to WT controls. also exhibited excessive colonic bleeding after 5 days, which correlated elevated white blood cells, neutrophils, lymphocytes blood, higher histopathology. Depletion commensal microflora antibiotic treatment demonstrated that inflammation animals microbiota-independent. Interestingly, microbiota-depleted appear have damage caused DSS since they become 10 days earlier than We investigated protective mechanism injection into knockout mice. Unexpectedly, mimicking secretion liver restoring did not lessen severity inflammation. This finding was supported transplantation bone marrow (BM) controls vice versa; BM became whereas most KO until administration. Since macrophages are only source known express Cp, transferred challenge. results demonstrate derived from contributes protection against DSS-induced