Evidences for vagus nerve in maintenance of immune balance and transmission of immune information from gut to brain in STM-infected rats

摘要: AIM: To determine whether Salmonella Typhimurium (STM) in gastrointestinal tract can induce the functional activation of brain, vagus nerve involves signaling immune information from to brain and how it influences function under natural infection condition. METHODS: Animal model rat was established by an intubation into stomach mimic condition bacteria infection. Subdiagphragmatic vagotomy performed some animals 28 days before The changes Fos expression visualized with immunohistochemistry technique hypothalamic paraventricular nucleus (PVN) superaoptic (SON) were counted. Meanwhile, percentage Mean Intensities Fluorescent (MIFs) CD4+ CD8+ T cells peripheral blood measured using flow cytometry (FCM), pathological ileum mesenteric lymph node observed HE stained sections. RESULTS: In bacteria-stimulated groups, inflammatory seen node. percentages decreased 42% ± 4.5% 34% 4.9% (P < 0.05) MIFs also 2.9 0.39 2.1 0.36 STM stimulation. All them proved that our STM-infection reliable. immunoreactive (Fos-ir) PVN SON increased significantly stimulation, 189 41 467 62 64 21 282 47 individually, which suggested induced brain. attenuated STM, 226 45 71 19 restored original level 44% 6.0% 0.05). addition, subdiagphragmatic itself (from 28% 3.0% 21% 5.9%, P 6.6 0.6 4.9 1.0, to1.4 0.34, Both manifested important role transmitting gut maintaining balance organism. CONCLUSION: Vagus does involve abdominal play maintenance organism.