Cdc16p, Cdc23p and Cdc27p form a complex essential for mitosis.

摘要: Abstract Cdc16p, Cdc23p and Cdc27p are all essential proteins required for cell cycle progression through mitosis in Saccharomyces cerevisiae. All three contain multiple tandemly repeated 34 amino acid tetratricopeptide repeats (TPRs). Using two independent assays, two-hybrid analysis vivo co-immunoprecipitation vitro, we demonstrate that Cdc16p, self associate interact with one another to form a macromolecular complex. A temperature sensitive mutation the most highly conserved TPR domain of results greatly reduced ability Cdc23p, but has no effect on interactions wild-type or Cdc16p. The specificity this indicates TPRs can mediate protein-protein may define an interaction yeast. conservation at least from yeast man suggests protein complex is wide range eukaryotes.