Analysis of islet regenerating (reg) gene polymorphisms in fibrocalculous pancreatic diabetes.

摘要: Fibrocalculous pancreatic diabetes (FCPD) is a form of associated with tropical chronic calcific pancreatitis, seen mostly in developing countries. FCPD likely to be multifactorial disease both environmental and genetic components. The reg 1A gene encodes protein regeneration islets has sequence identical that stone protein. Since we tested the hypothesis may result mutations coding regions gene. Restriction length polymorphisms (RFLPs) possible variants were studied by RFLP analysis, looking for single-stranded conformational (SSCPs) direct nucleotide sequencing. In 20 patients control subjects, no RFLPs detected using 10 restriction enzymes. 50 SSCP detected. Finally, sequencing from 30 did not show any differences published human sequence. conclusion, it seems unlikely region are common cause FCPD.