Rare circulating microRNAs as biomarkers of colorectal neoplasia.

摘要: Background MicroRNAs (miRNAs) are regulatory RNAs, stable in circulation, and implicated colorectal cancer (CRC) etiology progression. Therefore they promising as early detection biomarkers of neoplasia. However, many circulating miRNAs highly expressed blood cells, therefore may not be specific to neoplasia. Methods We selected 7 miRNA candidates with previously reported elevated expression adenoma tissue but low cells (“rare” miRNAs), 2 proposed biomarkers, 3 CRC. We conducted a colonoscopy-based case-control study including 48 polyp-free controls, 43 advanced adenomas, 73 non-advanced 8 CRC cases. from plasma were quantified by qRT-PCR. Correlations between levels, adjusted for age sex, assessed. used polytomous logistic regression estimate odds ratios (ORs) 95% confidence intervals quantifying the association levels case groups. also nonparametric receiver operating characteristic (ROC) analyses estimated area under curve (AUC). Results miRNAs high statistically significantly correlated one another. No associated or adenomas. Strong (ORs >5) significant associations observed 6 candidates, corresponding AUCs >0.5. Conclusions These candidate miRNAs, assayed qRT-PCR, probably unsuitable blood-based biomarkers. observed, primarily cells. These results suggest that rare will require new methods serve