Sacha Inchi Oil (Plukenetia volubilis L.), effect on adherence of Staphylococus aureus to human skin explant and keratinocytes in vitro.

摘要: Abstract Ethnopharmacological relevance Plukenetia volubilis L. (Euphorbiaceae) is a domesticated vine distributed from the high-altitude Andean rain forest to lowlands of Peruvian Amazon. Oil cold-pressed seeds, sold under commercial name Sacha Inchi (SIO) actually much in favour because it contains high percentage omega 3 and 6, hence used as dietary supplement. SIO also traditionally for skin care, order maintain softness, treatment wounds, insect bites infections, tropical context where frequently damaged. Aims study This was designed verify whether traditional use care would have any impact on Staphylococcus aureus growth adherence, S. involved many pathologies ( impetigo, folliculitis, furuncles subcutaneous abscesses) being one if main pathogens that can be found skin. Therefore, our objective assess bactericidal activity interference with adherence human explants keratinocyte cell line. Cytotoxicity cells determined. The compared coconut oil (CocO), which widely but has different unsaturated fatty acids contents. Materials methods Laboratory testing certified oil, determined antibacterial against radio labelled . Cytotoxic effects were measured XTT neutral red explants; phenol cytotoxic control. Adherence assays carried out by mixing H3-labelled bacteria explants, incubated oils 2 h before (to determine inhibition assimilated preventive effect) or after contact biological material detachment bacteria, curative effect). Residual radioactivity washings made possible intensity. Bactericidal effect colony counting trypticase soy agar. Results showed CocO, tested undiluted, not keratinocytes nor neither. more active antiadherent (preventive) than CocO keratinocytes. There no significant difference between (curative) both almost 5 times explants. Conclusion From concluded dermal safe efficient adherence. Our results tend support undiluted care.