Suberoylanilide hydroxamic acid promotes cardiomyocyte differentiation of rat mesenchymal stem cells

摘要: Abstract This study was to investigate the effect of suberoylanilide hydroxamic acid (SAHA), a histone deacetylase inhibitor, on cardiomyocyte differentiation bone marrow mesenchymal stem cells (MSCs) in vitro . Rat MSCs were isolated and induced differentiate into with SAHA or 5-azacytidine (5-aza, DNA methylation inhibitor) their combination. Following 7 days treatment, transcriptional expression cardiomyocyte-specific genes GATA4, NKx2.5, Mef2c dose-dependently increased up 15-fold increase mRNA levels over baseline. However, these only by 2–4 fold 5-aza-treated cells. After 4 weeks induction SAHA, cTnT protein content substantially 8-fold In contrast, minimal found When treated both 5-aza, same as those alone. These results indicate that effectively promotes rat much more potent inducer for cardiac then 5-aza. Our data also no synergistic antagonistic between 5-aza is present, acetylation, not demethylation, may be dominant mechanism determines MSCs.