Genetic Control of Immune Responses to Synthetic Polypeptides in Mice: Cellular Analysis of the Phenotypic Correction of the Ir-3 Gene Defect by Polyadenylic-Polyuridylic Acid

摘要: Antibody responses to the multichain synthetic polypeptide poly (L Tyr, L Glu)-poly Pro-poly Lys, (T, G)-Pro--L, and Pro-L portion of related polymer Phe, Pro--poly (Phe, are controlled by dominant, autosomal, non-H-2-linked, Ir-3 gene. SJL/J DBA/1 mice respective high low responder strains Pro-L. Injection with 300 µg poly(A)·poly(U) 24 hr after immunization either these immunogens resulted in an enhancement anti-Pro--L titers which were indistinguishable from those untreated or poly(A)·poly(U)-treated mice. In contrast, no effect on enhancing antibody DL Ala--poly G)-A-L, G) immunopotent region G)-Pro-L (immune H-2-linked Ir-1 gene) was obtained. The response also enhanced another strain, DBA/2. Thus, effects described here selective for phenotypic “correction” gene only, confirm that modes action genes distinct. Enhancement using G)-Pro-L, contains two regions, occurred at expense anti-(Phe, response. Limiting dilution patterns spleen marrow cells quantitatively qualitatively affected administering syngeneic hosts G)-Pro--L. No polynucleotide detected dilutions cells, nor thymocytes. lack thymocytes verified a two-step experimental design exposing antigen absence followed transferring thymus-derived irradiated test recipients. These results, as well (published elsewhere) Pro--L specific response, compatible hypothesis immunologic is expressed level cell-to-cell interaction, immunologically “corrects” expression this making interaction complementary cell types more effective.