Continuous abatement of methane coupled with ectoine production by Methylomicrobium alcaliphilum 20Z in stirred tank reactors: A step further towards greenhouse gas biorefineries

摘要: Abstract This study demonstrates for the first time feasibility of producing ectoine (a high added value osmoprotectant intensively used in cosmetic industry) during continuous abatement diluted emissions methane by Methylomicrobium alcaliphilum 20Z stirred tank reactors under non-sterile conditions. An increase NaCl concentration cultivation broth from 3 to 6% increased intra-cellular yield a factor 2 (from 16.5 37.4 mg (g biomass)−1), while stirring rates (600 rpm) entailed detrimental cellular stress and times lower yields (5.6 mg biomass)−1) compared process operation at 300 rpm. Cu2+ 0.05 25 μM enhanced (up elimination capacities 24.5 g m−3 h−1), did not enhance production but promoted excretion 20% total synthesized regardless rate. The results obtained culture hybridization with specific probe Mγ1004 showed that accounted more than 80% bacterial population most experimental runs. work confirmed technical new generation biorefineries based on CH4 using extremophile methanotrophs.