BCR-mediated apoptosis associated with negative selection of immature B cells is selectively dependent on Pten

摘要: The molecular basis of B cell receptor (BCR)-induced apoptosis during the negative selection immature cells is largely unknown. We use transitional that are highly susceptible to BCR-induced show Pten selectively required for BCR-mediated initiation mitochondrial death pathway. Specifically, deleting Pten, but not other pro-apoptotic molecules, abrogates BCR-elicited and improves viability in wild-type cells. further identify a physiologically significantly higher intracellular level cells, as compared mature which responsible low AKT activity propensity towards Restoration using constitutive form or reduction comparable with seen rescues from apoptosis. Thus, we provide evidence an essential mediator death, differential regulation levels determines whether BCR ligation promotes survival. Our findings valuable insight into mechanisms underlying clonal deletion