In vivo Tracking of Dendritic Cell using MRI Reporter Gene, Ferritin.

摘要: The noninvasive imaging of dendritic cells (DCs) migrated into lymph nodes (LNs) can provide helpful information on designing DCs-based immunotherapeutic strategies. This study is to investigate the influence transduction human ferritin heavy chain (FTH) and green fluorescence protein (GFP) genes inherent properties DCs, feasibility FTH as a magnetic resonance (MRI) reporter gene track DCs migration LNs. FTH-DCs were established by introduction GFP DC cell line (DC2.4) using lentivirus. changes in rate MRI signal decay (R2*) resulting from analyzed phantoms well popliteal LN mice after subcutaneous injection those hind limb foot pad multiple gradient echo sequence 9.4 T MR scanner. did not proliferation abilities DCs. expression co-stimulatory molecules (CD40, CD80 CD86) was similar that exhibited increased iron storage capacity, displayed significantly higher transverse relaxation compared phantom. LNs with negative contrast, leading high R2* both vivo ex T2*-weighted images On histological analysis subcapsular sinus zone LN, where they highly expressed CD25 bind stimulate cells. Our addresses an without alteration their properties. suggests FTH-based could be useful technique longitudinally monitor evaluate therapeutic efficacy DC-based vaccines.