Multiple immunoreplica Technique: screening for specific proteins with a series of different antibodies using one polyacrylamide gel.
作者： Roman P. Legocki , Desh Pal S. Verma
DOI: 10.1016/0003-2697(81)90577-7
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摘要: Abstract A simple method for immunological identification of proteins resolved electrophoretically is presented. Proteins from one polyacrylamide gel can be subjected to a series electrophoretic transfers nitrocellulose paper (partial “western-blots”), providing several replicas the gel. Each replica reacted with different antisera (at least three), where preceding antibody removed by treatment pH 2.2. The antigen-antibody complexes are visualized using 125I-Protein A. Reactivity and antigenic specificity immobilized on not affected repeated incubations low treatments. Identical size superimposable profiles detected antibodies allow precise localization particular polypeptides in original
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