Molecular cloning, sequencing and expression of human interferon-γ-inducible indoleamine 2,3-dioxygenase cDNA
作者： Wei Dai , Sohan L. Gupta
DOI: 10.1016/0006-291X(90)91666-G
关键词:
摘要: The antiproliferative action of human interferon (HuIFN)-gamma on cells and the inhibition intracellular pathogens, e.g. Toxoplasma gondii Chlamydia psittaci, is at least in part due to an induction indoleamine 2,3-dioxygenase (IDO) enzyme which degrades tryptophan, essential amino acid. A cDNA clone (called C42) was isolated from a library made poly(A)+ RNA obtained HuIFN-gamma-treated fibroblasts. Its nucleotide sequence revealed open reading frame coding for polypeptide 403 acids, but no homology with any known gene GenBank database found. Evidence indicating that this codes IDO: (i) Hybrid selected C42 specific IFN-gamma-treated coded vitro approximately 42 kD (reported size IDO, 40 kD) immunoprecipitated by monoclonal anti-IDO antibody not control antibody; (ii) transfection fibroblasts expression plasmid containing transcribed chicken beta-actin promoter led constitutive as well IDO activity. This will be useful studying role biological effects IFN-gamma, regulation IFN-gamma.
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