PBX1 is dispensable for neural commitment of RA-treated murine ES cells.
作者： Anne S. Jürgens , Mateusz Kolanczyk , Dietrich C. C. Moebest , Tomasz Zemojtel , Urs Lichtenauer
DOI: 10.1007/S11626-008-9162-5
关键词:
摘要: Experimentation with PBX1 knockout mice has shown that is necessary for early embryogenesis. Despite broad insight into function, little known about the underlying target gene regulation. Utilizing Cre-loxP system, we targeted a functionally important part of homeodomain through homozygous deletion exon-6 and flanking intronic regions leading to exon 7 skipping in embryonic stem (ES) cells. We induced vitro differentiation wild-type mutant ES cells by aggregation retinoic acid (RA) treatment compared their profiles expression at ninth day post-reattachment adhesive media. Our results indicate interactions HOX proteins DNA are dispensable RA-induced ability express neural genes point possible involvement regulation imprinted genes.
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L. T. Peltenburg, C. Murre, Engrailed and Hox homeodomain proteins contain a related Pbx interaction motif that recognizes a common structure present in Pbx. The EMBO Journal. ,vol. 15, pp. 3385- 3393 ,(1996) , 10.1002/J.1460-2075.1996.TB00704.X
Jorge Laborda, The role of the epidermal growth factor-like protein dlk in cell differentiation. Histology and Histopathology. ,vol. 15, pp. 119- 129 ,(2000) , 10.14670/HH-15.119
H. Hasegawa, S. C. Lian, W. E. Finkbeiner, A. S. Verkman, Extrarenal tissue distribution of CHIP28 water channels by in situ hybridization and antibody staining American Journal of Physiology-cell Physiology. ,vol. 266, ,(1994) , 10.1152/AJPCELL.1994.266.4.C893
Walter Zumkeller, Stefan Burdach, The Insulin-Like Growth Factor System in Normal and Malignant Hematopoietic Cells Blood. ,vol. 94, pp. 3653- 3657 ,(1999) , 10.1182/BLOOD.V94.11.3653.423K42_3653_3657
Melvin L. DePamphilis, Paul M. Wassarman, Guide to techniques in mouse development Academic Press Inc.. ,(1993)
Maria A. Morabito, Lori Redmond, Susan Hockfield, The Divergent Homeobox Gene PBX1 Is Expressed in the Postnatal Subventricular Zone and Interneurons of the Olfactory Bulb The Journal of Neuroscience. ,vol. 16, pp. 2972- 2982 ,(1996) , 10.1523/JNEUROSCI.16-09-02972.1996
Hitoshi Niwa, Jun-ichi Miyazaki, Austin G. Smith, Quantitative expression of Oct-3/4 defines differentiation, dedifferentiation or self-renewal of ES cells. Nature Genetics. ,vol. 24, pp. 372- 376 ,(2000) , 10.1038/74199
E.J. Robertson, R.S. Beddington, An assessment of the developmental potential of embryonic stem cells in the midgestation mouse embryo. Development. ,vol. 105, pp. 733- 737 ,(1989) , 10.1242/DEV.105.4.733
Licia Selleri, Kathryn S. E. Cheah, John L. R. Rubenstein, Stephen O’Gorman, Yakop Jacobs, Michael J. Depew, Michael L. Cleary, Sumit K. Chanda, Kwok Y. Tsang, Requirement for Pbx1 in skeletal patterning and programming chondrocyte proliferation and differentiation Development. ,vol. 128, pp. 3543- 3557 ,(2001) , 10.1242/DEV.128.18.3543
C P Chang, L Brocchieri, W F Shen, C Largman, M L Cleary, Pbx modulation of Hox homeodomain amino-terminal arms establishes different DNA-binding specificities across the Hox locus. Molecular and Cellular Biology. ,vol. 16, pp. 1734- 1745 ,(1996) , 10.1128/MCB.16.4.1734