Enzyme-immunoassay for the measurement of antigens using peroxidase conjugates.
作者： S AVRAMEAS , B GUILBERT
DOI: 10.1016/S0300-9084(72)80004-X
关键词:
摘要: Summary Human and rat IgG were labelled with peroxidase. Procedures established using these conjugates insoluble antibody immunoadsorbents for the quantitation of humoral IgG. The basic principles procedures are those already developed quantitative radio-immunoassay, enzyme activity measurement substituting radioactivity counting. allowed determination 10–200 ngs antigen. values obtained in unknown samples enzyme-immunoassays good agreement single radial immunodiffusion technique.
sci-hub.se 参考文章(5)
Stratis Avrameas, Immunoenzyme Techniques: Enzymes as Markers for the Localization of Antigens and Antibodies International Review of Cytology. ,vol. 27, pp. 349- 385 ,(1970) , 10.1016/S0074-7696(08)61250-4
Stratis Avrameas, Thérèse Ternynck, Biologically Active Water-insoluble Protein Polymers 1. THEIR USE FOR ISOLATION OF ANTIGENS AND ANTIBODIES Journal of Biological Chemistry. ,vol. 242, pp. 1651- 1659 ,(1967) , 10.1016/S0021-9258(18)96141-7
Dean Mann, John L. Fahey, Harriet Granger, Use of Insoluble Antibody for Quantitative Determination of Small Amounts of Immunoglobulin Journal of Immunology. ,vol. 102, pp. 618- 624 ,(1969)
B.K. Van Weemen, A.H.W.M. Schuurs, Immunoassay using antigen-enzyme conjugates FEBS Letters. ,vol. 15, pp. 232- 236 ,(1971) , 10.1016/0014-5793(71)80319-8
Thérèse Ternynck, Stratis Avrameas, Polyacrylamide-protein immunoadsorbents prepared with glutaraldehyde FEBS Letters. ,vol. 23, pp. 24- 28 ,(1972) , 10.1016/0014-5793(72)80274-6