Immunodiffusion analyses useful in clinical chemistry.

摘要: Publisher Summary Considering the proved value in biomedical research, immunodiffusion tests are startlingly underemployed for monitoring health and helping control disease. This chapter shows particular interest clinical chemistry to carefully selected examples of this lost information that could be most useful them. Immunodiffusion detect, measure, identify, or compare antigens antibodies better than other techniques, because they use each study specifically individually. Detecting one kind antigen among several with similar biochemical activities (e.g., enzymes) illustrates unique valuable capability immunodiffusion, using reagents (i.e., antibodies) tailored analytic objectives.Identifying is way identifying In addition antiserum specificity antigens, can identify It being used increasing immunoelectrophoresis 2-D electroimmunodiffusion (EID) analyses. There methods enhancing antigen-antibody reactions these include denaturing protein, intensifying complexes semispecifically specifically, staining proteins complexes, detecting by their own enzyme have been fixed gel antibodies, tagging radioactivity, fluorescence, enzymes, even cutting sections from look under an electron microscope. Citing specific, practical typical applications, discussion compares mechanisms merits commonly tests.