Identification of Treponema pedis as the predominant Treponema species in porcine skin ulcers by fluorescence in situ hybridization and high-throughput sequencing.

摘要: Skin lesions often seen in pig production are of great animal welfare concern. To study the potential role Treponema bacteria porcine skin ulcers, we investigated presence and distribution these organisms decubital shoulder ulcers (n=51) ear necroses (n=54) by fluorescence situ hybridization (FISH) high-throughput sequencing. In addition, two cases facial five other were included study. Samples from all 112 intact pigs without (n=14) screened FISH. Three different oligonucleotide probes targeting 16S rRNA used, specific for domain bacterium, spp. species T. pedis. Screening showed that each 35 (69%) 32 (59%) positive pedis was unequivocally, predominant typically constituting more than 90% treponemes a lesion, assessed visually microscopy. Altogether, demonstrated 69 71 lesions. We conclude frequently present abundant various pigs. The results this point toward an important as secondary bacterial infection especially severe chronic