6DMAP inhibition of early cell cycle events and induction of mitotic abnormalities.
作者： Marcella Simili , Paola Pellerano , Simona Pigullo , Gaia Tavosanis , Laura Ottaggio
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摘要: N-6 dimethylaminopurine (6DMAP) has been shown to induce aberrant mitosis in different cell types including Chinese hamster fibroblasts (CHEF/18), The mechanism of action and the cellular targets, however, are still not clear, We showed previously that CHEF/18 cells this compound inhibits DNA synthesis with a kinetic inhibition suggestive an effect on early events cycle, In paper we investigated which targets were affected by 6DMAP found that: (i) phosphorylation ribosomal protein S6 activation 70 kDa kinase (p70(S6k)) known be activated epidermal growth factor (EGF) keeping notion it is inhibitor; however vivo appears specific as MAP inhibited; (ii) drastically affects cytoskeletal components leading rapid morphological change most cells. These data, together findings dose range treatment time effective inducing micronuclei containing chromosomes same for inhibition, suggest disturbance G(1) signal transduction pathways may contribute abnormal mitosis.
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