Densitometric Validation and Optimisation of Polyphenols in Ocimum sanctum Linn by High Performance Thin-layer Chromatography.

摘要: Introduction Ocimum sanctum Linn (Sanskrit: Tulasi; family: Libiaceae), popularly known as holy basil or Ocimum teinufolium, is found throughout the semitropical and tropical parts of India. In Ayurveda, Tulasi has been well for its therapeutic potentials. Objective To optimise develop a standard method to quantify seven polyphenols simultaneously by HPTLC. Methods A three-level factor Box–Behnken statistical design was used optimisation, where extraction time (min), temperature (°C) methanol:water ratio (% v/v) are independent variables with dependent variable. The separation archived on silica-gel 60 F254 HPTLC plate using toluene:ethyl acetate:formic acid:methanol (3:3:0.8:0.2 v/v) mobile phase. Densitometric analysis carried out in absorbance mode at 366 nm. Results The quantification based peak area linear calibration curve concentration ranges 60–240, 20–200, 100–1600, 40–200, 200–1400, 10–160, 100–5000 ng/band caffeic acid, ellagic rutin, kaempferol, catechin, quercetin, eupalitin epicatechin respectively. validated purity, precision, accuracy, limit detection (LOD) (LOQ). Method specificity confirmed retention value visible spectra correlation marker compounds. Conclusions A newly developed simultaneous an Ayurvedic preparation O. sanctum. proposed simple, precise, specific, accurate, cost-effective, less consuming ability separate from other constituents. Copyright © 2015 John Wiley & Sons, Ltd.