Human tear peptide/protein profiling study of ocular surface diseases by SPE-MALDI-TOF mass spectrometry analyses
作者： Nerea González , Ibon Iloro , Javier Soria , Juan A. Duran , Alaitz Santamaría
DOI: 10.1016/J.EUPROT.2014.02.016
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摘要: Abstract The goal of this study was to investigate differences in tear peptidome/proteome profiles aqueous-deficient dry eye, Meibomian gland dysfunction and control individuals. Tears from 93 individuals were collected by capillary subjected solid-phase extraction followed MALDI-TOF for profiling analysis. Obtained spectra aligned variable penalty dynamic time warping (VPdtw) the resulting data analyzed using multivariate statistics. Comparative analyses revealed good performance VPdtw a high discrimination groups with correct assignment 89.3% twelve informative peaks. SDS–PAGE MALDI-TOF/TOF analysis allowed identification lipocalin-1 as biomarker candidate.
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Ricco Rakotomalala, TANAGRA : un logiciel gratuit pour l'enseignement et la recherche. EGC. pp. 697- 702 ,(2005)
Chris Bauer, Rainer Cramer, Johannes Schuchhardt, Evaluation of peak-picking algorithms for protein mass spectrometry. Methods of Molecular Biology. ,vol. 696, pp. 341- 352 ,(2011) , 10.1007/978-1-60761-987-1_22
Nerea González, Ibon Iloro, Juan A Durán, Félix Elortza, Tatiana Suárez, None, Evaluation of inter-day and inter-individual variability of tear peptide/protein profiles by MALDI-TOF MS analyses. Molecular Vision. ,vol. 18, pp. 1572- 1582 ,(2012)
Kelly K. Nichols, The international workshop on meibomian gland dysfunction: introduction. Investigative Ophthalmology & Visual Science. ,vol. 52, pp. 1917- 1921 ,(2011) , 10.1167/IOVS.10-6997
Christina Kramann, Nils Boehm, Katrin Lorenz, Nelli Wehrwein, Bernhard M. Stoffelns, Norbert Pfeiffer, Franz H. Grus, Effect of contact lenses on the protein composition in tear film: a ProteinChip study. Graefes Archive for Clinical and Experimental Ophthalmology. ,vol. 249, pp. 233- 243 ,(2011) , 10.1007/S00417-010-1456-0
Bon-Suk Koo, Do-Yeon Lee, Hyo-Shin Ha, Jae-Chan Kim, Chan-Wha Kim, Comparative analysis of the tear protein expression in blepharitis patients using two-dimensional electrophoresis. Journal of Proteome Research. ,vol. 4, pp. 719- 724 ,(2005) , 10.1021/PR0498133
Barbary Caffery, Elizabeth Joyce, Adrienne Boone, Allan Slomovic, Trefford Simpson, Lyndon Jones, Michelle Senchyna, Tear lipocalin and lysozyme in Sjögren and non-Sjögren dry eye. Optometry and Vision Science. ,vol. 85, pp. 661- 667 ,(2008) , 10.1097/OPX.0B013E318181AE4F
Éva Csősz, Péter Boross, Adrienne Csutak, András Berta, Ferenc Tóth, Szilárd Póliska, Zsolt Török, József Tőzsér, Quantitative analysis of proteins in the tear fluid of patients with diabetic retinopathy Journal of Proteomics. ,vol. 75, pp. 2196- 2204 ,(2012) , 10.1016/J.JPROT.2012.01.019
Michael Z. Wang, Brandon Howard, Michael J. Campa, Edward F. Patz, Michael C. Fitzgerald, Analysis of human serum proteins by liquid phase isoelectric focusing and matrix-assisted laser desorption/ionization-mass spectrometry. Proteomics. ,vol. 3, pp. 1661- 1666 ,(2003) , 10.1002/PMIC.200300513
J. Soria, J.A. Durán, J. Etxebarria, J. Merayo, N. González, R. Reigada, I. García, A. Acera, T. Suárez, Tear proteome and protein network analyses reveal a novel pentamarker panel for tear film characterization in dry eye and meibomian gland dysfunction. Journal of Proteomics. ,vol. 78, pp. 94- 112 ,(2013) , 10.1016/J.JPROT.2012.11.017