Immunohistochemical Expression of Stem Cell Markers during the Wound Healing Process of Cutaneous Burn
作者： Young Hee Choi , Min Gyu Kim , Dong-Hyun Ahn , Seong Jin Cho , Soo Hee Hong
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摘要: Purpose: A cutaneous wound healing requires a well-orchestrated integration of the complex biological and molecular events cell migration proliferation, extracellular matrix deposition, angiogenesis remodeling. Finally, skin regeneration is main goal. Stem cells are self-renewing multipotent progenitors with broadest developmental potential in given tissue at time. The aim this study was to examine role stem during process burn hairless mice by using immunohistochemical stainings (nestin, cytokeratin 15 CD31). Methods: Each mouse received 2 burns dorsal area applying metal stick heated boiling water. Burn sites were dressed duoderm. sacrificed 0, 2, 7, 14 21 days after burn. Histological findings expression for markers observed. Results: Nestin expressed stromal beneath epidermis, hair follices, dermal cysts endothelial cells. Cytokeratin epidermis except basal On 7 burn, regenerated didn't express 15. CD31 on amount nestin highest. Conclusion: Our results showed that may have various effects healing. before It likely other stimulate epithelial regeneration. act vascular (J Korean Surg Soc 2010;79:1-7)
koreascience.or.kr参考文章(13)
Haifeng Zhu, Xiaofei Wei, Ka Bian, Ferid Murad, Effects of nitric oxide on skin burn wound healing. Journal of Burn Care & Research. ,vol. 29, pp. 804- 814 ,(2008) , 10.1097/BCR.0B013E3181848119
Angela Webb, Amy Li, Pritinder Kaur, Location and phenotype of human adult keratinocyte stem cells of the skin. Differentiation. ,vol. 72, pp. 387- 395 ,(2004) , 10.1111/J.1432-0436.2004.07208005.X
Makoto Nogami, Tomoaki Hoshi, Tomomi Arai, Yoko Toukairin, Michiho Takama, Ichiro Takahashi, Morphology of lymphatic regeneration in rat incision wound healing in comparison with vascular regeneration. Legal Medicine. ,vol. 11, pp. 213- 218 ,(2009) , 10.1016/J.LEGALMED.2009.05.001
Rebecca J Morris, Yaping Liu, Lee Marles, Zaixin Yang, Carol Trempus, Shulan Li, Jamie S Lin, Janet A Sawicki, George Cotsarelis, Capturing and profiling adult hair follicle stem cells Nature Biotechnology. ,vol. 22, pp. 411- 417 ,(2004) , 10.1038/NBT950
Rebecca M Porter, Declan P Lunny, Patricia H Ogden, Susan M Morley, W H Irwin McLean, Alan Evans, Dolores L Harrison, Elizabeth L Rugg, E Birgitte Lane, K15 Expression Implies Lateral Differentiation within Stratified Epithelial Basal Cells Laboratory Investigation. ,vol. 80, pp. 1701- 1710 ,(2000) , 10.1038/LABINVEST.3780180
Silke Werner, Sabine Werner, Barbara Munz, Suppression of Keratin 15 Expression by Transforming Growth Factor β in Vitro and by Cutaneous Injury in Vivo Experimental Cell Research. ,vol. 254, pp. 80- 90 ,(2000) , 10.1006/EXCR.1999.4726
Fernando Benavides, Tatiana M. Oberyszyn, Anne M. VanBuskirk, Vivienne E. Reeve, Donna F. Kusewitt, The hairless mouse in skin research Journal of Dermatological Science. ,vol. 53, pp. 10- 18 ,(2009) , 10.1016/J.JDERMSCI.2008.08.012
L. Li, J. Mignone, M. Yang, M. Matic, S. Penman, G. Enikolopov, R. M. Hoffman, Nestin expression in hair follicle sheath progenitor cells. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 100, pp. 9958- 9961 ,(2003) , 10.1073/PNAS.1733025100
Y. Amoh, L. Li, K. Katsuoka, S. Penman, R. M. Hoffman, Multipotent nestin-positive, keratin-negative hair-follicle bulge stem cells can form neurons. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 102, pp. 5530- 5534 ,(2005) , 10.1073/PNAS.0501263102
Kimi Shimamura, Toshio Nakatani, Aiko Ueda, Junko Sugama, Mayumi Okuwa, Relationship between lymphangiogenesis and exudates during the wound-healing process of mouse skin full-thickness wound Wound Repair and Regeneration. ,vol. 17, pp. 598- 605 ,(2009) , 10.1111/J.1524-475X.2009.00512.X