Extensive cytokine analysis in synovial fluid of osteoarthritis patients.

摘要: Abstract Objective Osteoarthritis (OA) is a joint disease characterized by articular cartilage loss and afflicts many people worldwide. However, diagnostic methods treatment options remain limited are often low sensitivity efficacy. The focus of the present study was to identify proteomic biomarkers in synovial fluid improve diagnosis therapy OA patients. Methods: Antibody array technology utilized for protein expression profiling from 24 patients healthy persons. Results: Compared with persons, twenty proteins showed lower levels patients, while thirty had higher levels. Among these differential proteins, GITRL, CEACAM-1, FSH, EG-VEGF, FGF-4, PIGF, Cystatin EM NT-4 were found first time be differentially expressed OA. Bioinformatics analysis that most involved leukocytes events, some including IL-18, CXCL1, CTLA4, MIP-3b, CD40, MMP-1, THBS1, CCL11, PAI-1, BAFF, aggrecan, angiogenin follistatin located central positions protein–protein interaction (PPI) network. Conclusion: We speculate leukocyte proliferation migration may an important pathogenesis OA, which needs further validation. PPI network play more pivotal role newly identified novel targets therapy.