Plasminogen activators and their inhibitors in synovial fluids from normal, osteoarthritis, and rheumatoid arthritis knees.
作者： C Belcher , F Fawthrop , R Bunning , M Doherty
DOI: 10.1136/ARD.55.4.230
关键词:
摘要: OBJECTIVES: To establish baseline concentrations of plasminogen activators and their inhibitors in normal knee synovial fluids, to compare them with well characterised osteoarthritis (OA) rheumatoid arthritis (RA) fluids. METHODS: A total 26 subjects, 71 patients OA, 17 RA underwent aspiration. Patients OA were subclassified according presence nodal generalised (NGOA) fluid calcium pyrophosphate crystals. Clinical assessment inflammation (graded 0-6) was undertaken patients. Plasminogen activator (PA), inhibitor (PAI), urokinase-type PA receptor (uPAR) antigen determined by enzyme linked immunosorbent assay. The species PAs present sodium dodecyl sulphate-polyacrylamide gel electrophoresis. RESULTS: Concentrations all antigens (uPA, tissue-type (tPA), uPAR, PAI-1), significantly greater than OA; those normal. showed no direct association clinically assessed the knee. In associations age observed. Antigen tPA, uPAR) NGOA differed from other subclasses but did not appear vary between disease groups. predominant appeared have identity uPA. CONCLUSION: Because these compared cannot be used as a surrogate control studies PA/PAI system. Alteration system confirmed changes evident RA. finding different fluids further supports pathogenic mechanism this subset.
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