Determining hepatic triglyceride production in mice: comparison of poloxamer 407 with Triton WR-1339

摘要: Triglyceride (TG), a water-insoluble energy-rich lipid, is secreted by the liver as part of very low density lipoproteins (VLDLs) to supply energy extrahepatic tissues. Overproduction VLDL associated with increased risk cardiovascular heart disease; this has renewed an interest in factors that affect hepatic TG production. The production rate determined measuring temporal increases plasma under conditions which hydrolysis lipoprotein lipase (LPL) inhibited. nonionic detergent, Triton WR-1339 (Triton), commonly been used inhibit LPL for purpose. Triton, addition inhibition hydrolysis, properties have potential adversely influence metabolism. Another poloxamer 407 (P-407), also inhibits LPL. In these studies, we demonstrate P-407 comparable determination but without unwanted side effects Triton.