Real-time detection of rolling circle amplification products

摘要: Disclosed are compositions and methods for real-time detection of rolling circle amplification products. Real-time is that takes place during the reaction or operation. can be accomplished by, example, using fluorescent change probes and/or primers amplification. The signals proportional to amount product. multiply-primed in which replication a circular template primed at plurality sites on template. Multiply-primed RCA increases sensitivity singly-primed performed single primer (which hybridizes multiple target circle) (each hybridize site circle). Fluorescent involve fluorescence intensity wavelength based form conformation probe nucleic acid detected, assayed replicated.