Heteroduplex structures in 16S-23S rRNA intergenic transcribed spacer PCR products reveal ribosomal interoperonic polymorphisms within single Frankia strains.

摘要: Aims:  Detection of polymorphisms in intergenic transcribed spacer (ITS) 16S–23S rRNA within single Frankia strains. Methods and Results:  Polymorphisms the ITS were investigated single-colony subcultures seven isolates. Multiple ITS-polymerase chain reaction (PCR) bands detected solely isolates BMG5·5 BMG5·11. The slow-migrating ITS-PCR agarose gel electrophoresis profiles revealed to be heteroduplexes on basis their migration shift different electrophoretic matrices, southern hybridization single-strand DNA mung bean endonuclease digestion. Laser-scanned capillary two fragments differing length by three six nucleotide insertions/deletions strains BMG5·11, respectively. Sequence analysis cloned showed that strain differed presence four copies 3-bp tandem repeat 5′-TGG-3′. In 6-bp 5′-CTTGGG-3′. Conclusions:  We demonstrate occurrence 16S-23S rRNa strains. Significance Impact Study:  reported from Elaeagnus host group recognized as more flexible genus. Furthermore, we underscored applied interest BMG5·11 future ecological studies using molecular marker.