Occurrence and diversity of Frankia in Tunisian soil

摘要: There is a lack of studies on the occurrence and diversity Frankia in African soils, including those northern regions. The present study Tunisian soils an attempt to address this issue using AInus glutinosa, Elaeagnus angustifolia Casuarina glauca plant capturing bioassay 30 soil samples, followed by amplified 16S ribosomal DNA restriction pattern analysis (ARDRA). A total seven ARDRA haplotypes have been detected root actinorhizas that affiliated theoretical upon silico digestion selected RNA (rRNA) gene sequences retrieved from GeneBank confirmed their partial rRNA sequencing. F/aeagnus-compatible isolates were widespread form four Frankia, colonizing Elaeagnaceae Rhamnaceae two different phylogenetic subgroups. Alnus-compatible strains occurring subhumid area closely related AInus-Morella-compatible clustered haplotypes. Casuarina-compatible variability several arboreta. relatively wide opens perspective could be interesting reservoir for isolation new actinorhizal used as potential biofertilizers counteract progressive desertification which indeed crucial environmental problem Northern Africa.