The more basic isoform of eEF1A relates to tumour cell phenotype and is modulated by hyper-proliferative/differentiating stimuli in normal lymphocytes and CCRF-CEM T-lymphoblasts.
作者： Bruna Scaggiante , Barbara Dapas , Gabriele Pozzato , Gabriele Grassi
DOI: 10.1002/HON.2022
关键词:
摘要: The elongation factor 1A proteins (eEF1A1/A2) are known to play a role in tumours. We previously found that more basic isoform of eEF1A (MBI-eEF1A) is present the cytoskeletal/nuclear-enriched extracts CCRF-CEM T-lymphoblasts but not those normal lymphocytes. To obtain deeper knowledge about MBI-eEF1A biology, we investigate from which proteins, eEF1A1 or eEF1A2, originates and possibility its appearance can be modulated by differentiated proliferative cell status. lymphocytes were cultured with without differentiation/pro-proliferative stimuli (Phorbol 12-Myristate 13-Acetate (PMA) alone combination phytohaemagglutinin (PHA) PMA, respectively), presence evaluated together eEF1A1/A2 mRNAs. Our data indicate may derive as eEF1A2 expressed Moreover, inducible upon hyper-proliferative application; CCRF-CEM, abrogated PMA-induced differentiation. Finally, have functional hyper-proliferating/tumour cells disappearance reduces growth PHA/PMA-stimulated presented suggest related oncogenic phenotype, rising use target for novel therapeutic strategies. Copyright © 2012 John Wiley & Sons, Ltd.
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