Highly enhancing the characteristics of immobilized thermostable β-glucosidase by Zn2+
作者： Xuejia Shi , Linguo Zhao , Jianjun Pei , Lin Ge , Pengwei Wan
DOI: 10.1016/J.PROCBIO.2018.01.004
关键词:
摘要: Abstract The thermostable GH3 β-glycosidase (Tpebgl3) from Thermotoga petrophila DSM 13995 was immobilized on macroporous resin NKA-9 modified with polyethylenimine (PEI) and glutaraldehyde (named NKA-9II). properties of NKA-9II were as follows: the optimal conditions same that free enzyme (pH 5.0; 90 °C), highest activity cellobiose substrate approached 1.7 U/g; thermostability, pH stability glucose tolerance greatly improved; residual 68% initial at end 10 repeated cycles. Moreover, it found 2 mM Zn2+ increased relative to 192% 199% p-nitrophenyl-β- d -glucopyranoside (pNPG) substrates, respectively. Meanwhile, could improve reusability, high-temperature stability, NKA-9II. In particular, 84% retained, which 21% higher than metal ion after incubation 85 °C for 7 h; when concentration 400 mM, Tpebgl3 completely inactivated, maintained 63% its activity, 19.5% in absence Zn2+.
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