Immobilization of Yarrowia lipolytica Lipase on Macroporous Resin Using Different Methods: Characterization of the Biocatalysts in Hydrolysis Reaction.
作者： Jingjing Sun , Yiling Chen , Jun Sheng , Mi Sun
DOI: 10.1155/2015/139179
关键词:
摘要: To improve the reusability and organic solvent tolerance of microbial lipase expand application (hydrolysis, esterification, transesterification), we immobilized marine using different methods determined properties lipases. Considering activity cost lipase, concentration was fixed at 2 mg/mL. The optimal temperature lipases 40°C 5°C higher than free lipase. activities were much alkaline pH (more 50% 12). lost most (35.3%) retained more 46.4% their initial after 3 h heat treatment 70°C. At pH, stable 60% residue 11 for 3 h). Immobilized 80% 5 cycles increased enzyme 108.7%) in tert-butanol. Immobilization which improved provided a chance to system expanded range catalyze hydrolysis esterification harsh condition.
hindawi.com
core.ac.uk参考文章(45)
Sébastien Elgue, Annelyse Conté, Alain Marty, Jean-Stéphane Condoret, Continuous lipase esterification using process intensification technologies Journal of Chemical Technology & Biotechnology. ,vol. 89, pp. 1590- 1598 ,(2014) , 10.1002/JCTB.4247
Lilin Li, Wei Du, Dehua Liu, Li Wang, Zebo Li, Lipase-catalyzed transesterification of rapeseed oils for biodiesel production with a novel organic solvent as the reaction medium Journal of Molecular Catalysis B-enzymatic. ,vol. 43, pp. 58- 62 ,(2006) , 10.1016/J.MOLCATB.2006.06.012
Tien-Chieh Hung, R Giridhar, Shao-Hua Chiou, Wen-Teng Wu, Binary immobilization of Candida rugosa lipase on chitosan Journal of Molecular Catalysis B: Enzymatic. ,vol. 26, pp. 69- 78 ,(2003) , 10.1016/S1381-1177(03)00167-X
Dasciana S Rodrigues, Adriano A Mendes, Wellington S Adriano, Luciana RB Gonçalves, Raquel LC Giordano, None, Multipoint covalent immobilization of microbial lipase on chitosan and agarose activated by different methods Journal of Molecular Catalysis B-enzymatic. ,vol. 51, pp. 100- 109 ,(2008) , 10.1016/J.MOLCATB.2007.11.016
Audrey Sassolas, Loïc J. Blum, Béatrice D. Leca-Bouvier, Immobilization strategies to develop enzymatic biosensors Biotechnology Advances. ,vol. 30, pp. 489- 511 ,(2012) , 10.1016/J.BIOTECHADV.2011.09.003
Urszula Guzik, Katarzyna Hupert-Kocurek, Danuta Wojcieszyńska, Immobilization as a Strategy for Improving Enzyme Properties-Application to Oxidoreductases Molecules. ,vol. 19, pp. 8995- 9018 ,(2014) , 10.3390/MOLECULES19078995
Panagiota-Yiolanda Stergiou, Athanasios Foukis, Michalis Filippou, Maria Koukouritaki, Maria Parapouli, Leonidas G Theodorou, Efstathios Hatziloukas, Amalia Afendra, Ashok Pandey, Emmanuel M Papamichael, Advances in lipase-catalyzed esterification reactions. Biotechnology Advances. ,vol. 31, pp. 1846- 1859 ,(2013) , 10.1016/J.BIOTECHADV.2013.08.006
Emese Biró, Ágnes Sz. Németh, Csaba Sisak, Tivadar Feczkó, János Gyenis, Preparation of chitosan particles suitable for enzyme immobilization Journal of Biochemical and Biophysical Methods. ,vol. 70, pp. 1240- 1246 ,(2008) , 10.1016/J.JPROT.2007.11.005
Shengdong Zhu, Yuanxin Wu, Ziniu Yu, Immobilization of Candida rugosa lipase on a pH-sensitive support for enantioselective hydrolysis of ketoprofen ester Journal of Biotechnology. ,vol. 116, pp. 397- 401 ,(2005) , 10.1016/J.JBIOTEC.2004.12.012
Anny Manrich, Célia MA Galvão, Charles DF Jesus, Roberto C Giordano, Raquel LC Giordano, None, Immobilization of trypsin on chitosan gels: use of different activation protocols and comparison with other supports. International Journal of Biological Macromolecules. ,vol. 43, pp. 54- 61 ,(2008) , 10.1016/J.IJBIOMAC.2007.11.007