Cell surface and cellular debris-associated heat-stable lipolytic enzyme activities of the marine algaNannochloropsis oceanica

摘要: AbstractMicrobial surface display of lipases can be effectively employed for the development whole-cell biocatalysts industrial bioconversions. In present work, we report first time presence thermostable lipolytic enzyme activities against p-nitrophenyl laurate, both on cell and cellular debris fraction marine microalga Nannochloropsis oceanica (strain CCMP1779). Whole cell-associated activity (WCLA) shows a 2.5-fold stimulation after heat treatment at 100 °C 60 min, while respective is retained 15 min. contrast, renders soluble disrupted cells inactive. The progress curve debris-associated lipase biphasic levels off very fast. Treatment with surfactants SDS, Triton X-100 CHAPS, which are known to inhibit in various degrees, results loss bound (CDLA). highest wh...