REGULATION AND COORDINATION OF PURINE AND PYRIMIDINE BIOSYNTHESES IN YEAST. I. REGULATION OF PURINE BIOSYNTHESIS AND ITS RELATION TO TRANSIENT CHANGES IN INTRACELLULAR NUCLEOTIDE LEVELS.

摘要: The control of purine biosynthesis in a yeast mutant deficient for uracil, adenine, and histidine has been studied vivo. adenine mutation causes accumulation aminoimidazole ribotide the cells. curve relating steady-state nucleotide level cell to rate synthesis de novo synthetic pathway determined. Control depends on feedback mechanism involving end-product inhibition. transient responses pool changes input have studied. Under certain conditions overshoots when shifting from one another. Transient levels are followed by inverse attempted synthesis. A study specific intracellular nucleotides suggests that inosinic acid controls response nucleic was implications regulation discussed.