A nonselective cation channel in rat liver cells is activated by membrane stretch

摘要: A 16-pS channel was studied using patch-clamp electrophysiology in freshly dissociated rat liver cells and hepatoma cells. The found to be cation selective permeable Na+, K+, Ca2+. Its gating unaffected by addition of the calcium ionophore A23187 (5 microM) presence extracellular Ca2+ (2 mM). blockers, nifedipine, verapamil, lanthanum, failed inhibit channel. activated stretch, applied as suction interior patch pipette, cell swelling, induced hypotonic shock or organic solute uptake (10 mM L-alanine). Channel activation swelling transient, lasting approximately 1 min. An elevation cytosolic evoked shock, measured fluorescent indicator indo-1/AM. This change intracellular concentration dependent on Inasmuch time course for this response corresponded that activation, it is likely stimulated influx through stretch-activated To determine role regulatory volume decrease (RVD), changes after were a Coulter counter. RVD slightly but significantly inhibited depletion On basis these results proposed channels permit transient Ca2+, which turn acts trigger ion conductance cytoskeletal components involved regulation.