Evaluation of INNO-LIA Syphilis Assay as a Confirmatory Test for Syphilis

摘要: We evaluated the sensitivity and specificity of a new confirmatory test for treponemal antibodies, INNO-LIA Syphilis (Innogenetics NV, Ghent, Belgium), on large number sera from clinical laboratory. This multiparameter line immunoassay (LIA) uses recombinant synthetic polypeptide antigens derived Treponema pallidum proteins. In single-blinded cross-sectional retrospective study, 289 seronegative sera, 219 seropositive 23 with an indeterminate serological status syphilis were analyzed. All tested by T. hemagglutination assay (TPHA), immunoglobulin (IgG)-fluorescent absorption (IgG-FTA-ABS), Venereal Disease Research Laboratory (VDRL) test. addition, some samples analyzed 19S-IgM-FTA-ABS test, enzyme (IgM-EIA), MarDx immunoblotting assay. Based consensus diagnosis conventional serology, all classified as positive, negative, or indeterminate, results compared findings The LIA 100% (219 219) 99.3% (286 288), respectively. Compared to TPHA IgG-FTA-ABS, gave significantly higher (P = 0.021 P < 0.0001, respectively) correct than either other two tests. is useful because it increases reliability respect current techniques.