Polypeptides of Treponema pallidum: progress toward understanding their structural, functional, and immunologic roles. Treponema Pallidum Polypeptide Research Group.

摘要: Treponema pallidum subsp. pallidum, the spirochete that causes syphilis, is unusual in a number of respects, including its small genome size, inability to grow under standard vitro culture conditions, microaerophilism, apparent paucity outer membrane proteins, structurally complex periplasmic flagella, and ability evade host immune responses cause disease over period years decades. Many these attributes are related ultimately protein content. Our knowledge activities, structure, immunogenicity proteins has been expanded by application recombinant DNA, hybridoma, structural fractionation techniques. The purpose this monograph summarize correlate new information using two-dimensional gel electrophoresis, monoclonal antibody reactivity, sequence data, other properties as bases polypeptide identification. profiles T. subspecies causing yaws, endemic syphilis virtually indistinguishable but differ considerably from those treponemal species. Among most abundant polypeptides group lipoproteins unknown function appear be important response during syphilitic infection. flagella spirochetes unique with regard their content ultrastructure, well location. They composed three core (homologous members eubacterial flagellin family) single, unrelated sheath protein; functional significance arrangement not understood at present. Although bacterium contains chaperonins GroEL DnaK, control heat shock regulon they organisms. Studies indicate many may useful for immunodiagnosis immunoprotection. Future goals research include continued elucidation locations identification characterization low-abundance further study immunoprotective immunodiagnostic potential clarification roles pathogenesis. Images