Overexpression of the Wilms' tumor gene WT1 in colorectal adenocarcinoma
作者： Yusuke Oji , Hirofumi Yamamoto , Masaya Nomura , Yoko Nakano , Ai Ikeba
DOI: 10.1111/J.1349-7006.2003.TB01507.X
关键词:
摘要: Expression of the Wilms' tumor gene WT1 was examined in 59 cases colorectal adenocarcinoma to examine involvement tumorigenesis. Quantitative real-time reverse transcription-polymerase chain reaction (RT-PCR) showed that mRNA expressed range from 7.2 x 10(-5) 4.9 10(-1) levels (WT1 expression level K562 leukemic cells defined as 1.0) all (100%) 28 examined, and were higher 20 (71%) compared those normal-appearing mucosal tissues examined. Immunohistochemical analysis using an anti-WT1 antibody performed on 46 (15 with 31 newly collected cases), protein detected 41 (89%) cases. The direct sequencing genomic DNA no mutations any 10 exons 5 different adenocarcinomas. These results may indicate important role wild-type tumorigenesis adenocarcinoma.
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