Amplification and Elevated Expression of c-myc in a Chemically Induced Mouse Colon Tumor

摘要: Abstract The amplification and level of expression four cellular oncogenes was investigated in dimethylhydrazine-induced Mouse Colon Tumor 36 Corbett et al. [Corbett, T. H. Griswold, D. P., Jr., Roberts, B. J., Peckham, J. C., Schabel, F. M., Jr. Cancer (Phila.), 40: 2660–2680, 1977], which has been used to evaluate potential chemotherapeutic agents. A 30- 40-fold c- myc observed the tumor DNA as compared normal colonic mucosa liver. No changes were number copies mos, erb B , or bas . Digestion with three different restriction enzymes demonstrated that there no major alteration genomic structure amplified DNA. RNA 10-fold higher both total polyadenylated from when mucosa. Probes specific for exons hybridization experiments all present mRNA.