Liquid chromatographic determination of free and conjugated 3-methoxy-4-hydroxyphenylethylene glycol with wide-ranging substances related to monoamine metabolism

摘要: Abstract A simple and sensitive procedure was developed for the simultaneous determination of substances metabolically related to monoamine transmitters including 3-methoxy-4-hydroxyphenylethylene glycol (MOPEG) in dissected brain regions rats using high-performance liquid chromatography combined with electrochemical detection. The tissue sample homogenized HCl solution. homogenate divided into two portions, which one used assay MOPEG after enzymatic hydrolysis sulfatase. butanol extraction process performed on remaining portion obtain transmitters, precursor amino acids, acidic metabolites. monoamines acids were finally recovered solution, while metabolites shifted alkaline buffer from organic layer. basic neutral separated a 0.1 m sodium citrate/citric acid system (pH 4.0) containing 1% tetrahydrofuran, ones 0.075 3.5) 10% methanol, 12% acetic acid. steady-state concentrations three (noradrenaline, dopamine, 5-hydroxytryptamine) determined together their precursors Changes these examined various drugs, effects had been previously confirmed. changes reflected putative drug demonstrated that applicable regional substances.