Thymosin α1 is a time and dose-dependent antagonist of dexamethasone-induced apoptosis of murine thymocytes in vitro
作者： Christian A Baumann , Mahnaz Badamchian , Allan L Goldstein
DOI: 10.1016/S0192-0561(00)00065-5
关键词:
摘要: It is well established that glucocorticoid hormones induce apoptosis in immature developing thymocytes. Thymocyte can be modulated by growth factors, anti-oxidants and adhesion receptors. We have previously demonstrated thymosin alpha1 (Talpha1) antagonizes dexamethasone-induced of CD4+CD8+ In the present study, we further characterize dose time dependence Talpha1's antagonism thymocyte apoptosis. Talpha1 effective at concentrations ranging from 2 to 100 microg/10(6) pre-treatment necessary achieve its anti-apoptotic activity. provides temporary protection thymocytes slowing dexamethasone's apoptotic activity up 12 h post dexamethasone treatment. Additionally, not sensitive cycloheximide treatment, suggesting independent protein synthesis. Finally, unable antagonize induced reactive oxygen species, H2O2, occurs early stages apoptosis, prior production species. This evidence suggests may provide a mechanism transiently extend life during thymic selection.
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