5′-Nucleotidase in the Detection of Increased Activity of the Liver Form of Alkaline Phosphatase in Serum

摘要: 5′-Nucleotidase (5′-ribonucleotide phosphohydrolase; EC 3.1.3.5; 5NT), an intrinsic membrane glycoprotein present as ectoenzyme in a wide variety of mammalian cells, hydrolyzes 5′-nucleotides to their corresponding nucleosides (1). Despite its ubiquitous distribution, serum concentrations 5NT appear reflect hepatobiliary disease with considerable specificity (2). Dixon and Purdom (3) first observed that was clinically useful for differential diagnosis osseous diseases, the enzyme activity being increased only diseases. This finding has been confirmed by several authors (4)(5)(6)(7). Assay may have value addition measurement nonspecific total alkaline phosphatase (AP) patients suspected (8)(9)(10)(11)(12), abnormal is routinely interpreted some clinicians evidence hepatic origin AP serum. However, direct comparison liver form (L-AP) clinical setting not reported (13). The aim this …