Monoclonal antibodies to type IV collagenase recognize a protein with limited sequence homology to interstitial collagenase and stromelysin
作者： Matti Höyhtyä , Taina Turpeenniemi-Hujanen , William Stetler-Stevenson , Henry Krutzsch , Karl Tryggvason
DOI: 10.1016/0014-5793(88)81365-6
关键词:
摘要: Type IV collagenase is a metalloproteinase associated with metastatic tumor cells. It specifically cleaves the triple helical basement membrane (type IV) collagen molecule at single site. Monoclonal antibodies which block activity of human type were developed and used to purify this antigen. The purified was partially sequenced following cyanogen bromide trypsin cleavage. amino acid sequence fragments revealed region homologous interstitial stromelysin. However, several sequences in identified are distinct from latter. Polyclonal raised against synthetic peptide derived such sequence. Following affinity purification, recognized denatured Western immunoblotting. These data indicate that member general family metalloproteinases.
core.ac.uk
elsevier.com
sci-hub.se 参考文章(17)
S E Whitham, G Murphy, P Angel, H J Rahmsdorf, B J Smith, A Lyons, T J R Harris, J J Reynolds, P Herrlich, A J P Docherty, Comparison of human stromelysin and collagenase by cloning and sequence analysis Biochemical Journal. ,vol. 240, pp. 913- 916 ,(1986) , 10.1042/BJ2400913
G Fastelli, L A Liotta, S Garbisa, A Negro, M Naturale, D Daga-Gordini, M Ballin, G Semenzato, Transient expression of type IV collagenolytic metalloproteinase by human mononuclear phagocytes. Journal of Biological Chemistry. ,vol. 261, pp. 2369- 2375 ,(1986) , 10.1016/S0021-9258(17)35946-X
T Salo, L A Liotta, K Tryggvason, Purification and characterization of a murine basement membrane collagen-degrading enzyme secreted by metastatic tumor cells. Journal of Biological Chemistry. ,vol. 258, pp. 3058- 3063 ,(1983) , 10.1016/S0021-9258(18)32829-1
G I Goldberg, S M Wilhelm, A Kronberger, E A Bauer, G A Grant, A Z Eisen, Human fibroblast collagenase. Complete primary structure and homology to an oncogene transformation-induced rat protein. Journal of Biological Chemistry. ,vol. 261, pp. 6600- 6605 ,(1986) , 10.1016/S0021-9258(19)84605-7
L I Fessler, K G Duncan, J H Fessler, T Salo, K Tryggvason, Characterization of the procollagen IV cleavage products produced by a specific tumor collagenase. Journal of Biological Chemistry. ,vol. 259, pp. 9783- 9789 ,(1984) , 10.1016/S0021-9258(17)42768-2
U P Thorgeirsson, T Turpeenniemi-Hujanen, J E Williams, E H Westin, C A Heilman, J E Talmadge, L A Liotta, NIH/3T3 cells transfected with human tumor DNA containing activated ras oncogenes express the metastatic phenotype in nude mice. Molecular and Cellular Biology. ,vol. 5, pp. 259- 262 ,(1985) , 10.1128/MCB.5.1.259
L. A. Liotta, K. Tryggvason, S. Garbisa, Ian Hart, C. M. Foltz, S. Shafie, Metastatic potential correlates with enzymatic degradation of basement membrane collagen. Nature. ,vol. 284, pp. 67- 68 ,(1980) , 10.1038/284067A0
Lance A. Liotta, Karl Tryggvason, Spiridione Garbisa, Pamela Gehron Robey, Shigeto Abe, Partial purification and characterization of a neutral protease which cleaves type IV collagen. Biochemistry. ,vol. 20, pp. 100- 104 ,(1981) , 10.1021/BI00504A017
G. KÖHLER, C. MILSTEIN, Continuous cultures of fused cells secreting antibody of predefined specificity Nature. ,vol. 256, pp. 495- 497 ,(1975) , 10.1038/256495A0
Rupert TIMPL, Hanna WIEDEMANN, Vera DELDEN, Heinz FURTHMAYR, Klaus KUHN, A Network Model for the Organization of Type IV Collagen Molecules in Basement Membranes FEBS Journal. ,vol. 120, pp. 203- 211 ,(1981) , 10.1111/J.1432-1033.1981.TB05690.X