MicroRNA Let-7b inhibits keratinocyte migration in cutaneous wound healing by targeting IGF2BP2

摘要: Wound healing is a complex process which involves proliferation and migration of keratinocyte for closure epidermal injuries. A member microRNA family, let-7b, has been expressed in mammalian skin, but its exact role still not knowledge. Here, we showed that let-7b regulates by targeting the insulin-like growth factor IGF2BP2. Overexpression led to reduced HaCaT cell migration, while knockdown resulted enhanced migration. Furthermore, was decreased during wound wild-type mice, us construct transgenic mice with overexpression skin. The re-epithelialization epidermis healing. Using bioinformatics prediction software reporter gene assay, found IGF2BP2 target contributes Introduction an expression vector also rescued let-7b-induced deficiency, confirms important regulation. Our findings suggest significantly delayed possibly due reduction restraints skin