The isolation of molecular genetic markers for the identification of sex

摘要: Abstract It is difficult to identify sex in many species of eukaryotic organism. This can considerably impede research into their biology. Fortunately, one often possesses a unique chromosome termed Y or W. When DNA markers are available for these chromosomes, then identification becomes straightforward. We describe technique that facilitates the isolation such markers. The procedure makes use low-stringency PCR screen randomly selected primers ability amplify sex-specific loci.