Genetic Strategies for Identifying New Drug Targets

摘要: Genetic strategies have yet to come into their own as tools for antibiotic development. While holding a lot of initial promise, they only recently started bear fruit in the quest new drug targets. An ever-increasing body knowledge is showing that genetics can lead significant improvements success and efficiency discovery. Techniques such high-frequency transposon mutagenesis expression modulation matured been applied successfully not identification characterization targets, but also validation tractable weaknesses bacteria. Past experience shows choosing targets must rely on gene essentiality alone, rather needs incorporate system whole. The ability manipulate genes key ensuring we understand entire set processes are affected by treatment. Focusing exacerbating these perturbations, together with which resistance has occurred—both enabled genetic approaches—may point us toward successful development combination therapies engineered based underlying biology.