Electrochemically Modified Carbon and Chromium Surfaces for AFM Imaging of Double‐Strand DNA Interaction with Transposase Protein

摘要: Carbon and chromium surfaces were modified by electrochemical reduction of a diazonium salt formed in situ from the sulfanilic acid. The organic layer was activated phosphorus pentachloride (PCl5) to form benzene sulfonil chloride (ArSO2Cl). An study blocking effect activity this surface carried out on carbon electrode. completed X-ray photoelectron spectroscopy atomic force microscopy characterize formation compact monolayer (0.8 nm height roughness 0.2–0.3 nm). compactness allowed us affix length dsDNA with aim analyzing complex between protein. interaction transposase protein its target investigated. direct imaging nucleoproteic considered herein gives new insights comprehension transposase–DNA agreement biochemical data.